Matrices listed below were spiked with certain level of IgG and the recovery rates were calculated by comparing the measured value to the expected amount of IgG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-101
95
EDTA plasma(n=5)
89-99
94
heparin plasma(n=5)
90-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
93-100%
85-93%
EDTA plasma(n=5)
84-95%
83-99%
86-100%
heparin plasma(n=5)
84-98%
85-99%
84-99%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IgG ELISA Kit allows for the in vitro quantitative determination of IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.