Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IgA1 and the recovery rates were calculated by comparing the measured value to the expected amount of IgA1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
96-101
98
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
90-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
85-92%
94-103%
EDTA plasma(n=5)
85-99%
83-96%
89-97%
heparin plasma(n=5)
81-93%
82-99%
87-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IgA1ELISA Kit allows for the in vitro quantitative determination of IgA1 concentrations in serum, plasma, tissue homogenates and other biological fluids.