Matrices listed below were spiked with certain level of Iapp and the recovery rates were calculated by comparing the measured value to the expected amount of Iapp in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
97
EDTA plasma(n=5)
87-105
99
heparin plasma(n=5)
85-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Iapp and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Iapp ELISA Kit allows for the in vitro quantitative determination of Iapp concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Iapp(Islet amyloid polypeptide) ELISA Kit