Rat hypoxia-inducible factor 1alpha,HIF-1alpha ELISA Kit

Rat hypoxia-inducible factor 1alpha,HIF-1alpha ELISA Kit

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Mfr.No.

CSB-E08540r

Alternative Names

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, ARNT interacting protein, hypoxia-inducible factor 1 alpha isoform I.3, hypoxia-inducible factor 1, alpha subunit, hypoxia-inducible factor 1, alpha, hypoxia-inducible factor 1alpha(HIF-1alpha), hypoxia-inducible factor 1 alpha(HIF-1 alpha)

Reactivity

Rat

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse pAMPK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	95	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse pAMPK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-101
1:2	Average %	102
	Range %	99-104
1:4	Average %	89
	Range %	87-92
1:8	Average %	94
	Range %	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.152	2.233	2.193	2.031
5	1.378	1.405	1.392	1.230
2.5	0.806	0.858	0.832	0.670
1.25	0.488	0.452	0.470	0.308
0.625	0.305	0.306	0.306	0.144
0.312	0.249	0.257	0.253	0.091
0.156	0.212	0.202	0.207	0.045
0	0.158	0.165	0.162

Species

Rattus norvegicus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

UniProt ID	O35800
Antigen	hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Applications

ELISA

Research Area

HIF1A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508142	CSB-E08540r	96T	$856	Add to Cart
BHE10508142	CSB-E08540r	5x96T	$4108.80	Add to Cart
BHE10508142	CSB-E08540r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.