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Home / ELISA Kit

Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit

SKU : BHE13700950

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.

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Mfr.No.	KTE100731
Reactivity	Rat
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1A.
Alternative Names	HIF1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; hypoxia-inducible factor 1; alpha
Detection Method	Colorimetric
Kit Components	Rat Hypoxia-inducible factor 1-alpha microplate Rat Hypoxia-inducible factor 1-alpha standard Rat Hypoxia-inducible factor 1-alpha detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat HIF1A. No significant cross-reactivity or interference between Rat HIF1A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
Notes	This Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1A in samples. An antibody specific for HIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	29560

Applications	ELISA
Applications Notes	This Rat Hypoxia-inducible factor 1-alpha (HIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate HIF1A in samples. An antibody specific for HIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHIF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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