Rat Hypoxanthine-Guanine Phosphoribosyltransferase

Mfr.No.

E1802Ra

Sensitivity

0.081ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Hypoxanthine-guanine Phosphoribosyltransferase (also known as HPRT1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HGPRT;HGPRTase;HPRT 1;HPRT1;Hypoxanthine-guanine phosphoribosyltransferase

Molecular Weight

24477

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1802Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.7	0.38	5.7
Intra-Assay	2	18	19.4	0.69	3.6
Intra-Assay	3	18	17.1	1.12	6.6

Background

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).

Protocol

These standard curves of E1802Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
36ng/ml	2.055	2.16	2.115
	2.264
18ng/ml	1.252	1.214	1.17
	1.176
9ng/ml	0.808	0.733	0.688
	0.658
4.5ng/ml	0.433	0.467	0.423
	0.502
2.25ng/ml	0.189	0.196	0.152
	0.203
0ng/ml	0.057	0.044	0
	0.032

Gene ID	3251
UniProt ID	P27605

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113003	E1802Ra	48T	$320		Add to Cart
BHE12113003	E1802Ra	96T	$458		Add to Cart

Rat Hypoxanthine-Guanine Phosphoribosyltransferase - HPRT1 ELISA Kit

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