Rat Huwe1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit

Mfr.No.

ER0662

Alternative Names

Huwe1 ELISA Kit, HECT ELISA Kit, UBA and WWE domain-containing protein 1 ELISA Kit, Upstream regulatory element-binding protein 1 ELISA Kit, URE-B1 ELISA Kit, URE-binding protein 1 ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.781-50ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Huwe1 and the recovery rates were calculated by comparing the measured value to the expected amount of Huwe1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	92-101	97
EDTA plasma(n=5)	90-100	95
heparin plasma(n=5)	96-101	98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Huwe1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-105%	89-101%	87-103%
EDTA plasma(n=5)	83-96%	89-100%	86-101%
heparin plasma(n=5)	80-100%	81-98%	86-98%

Sensitivity

< 0.469ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Huwe1/HECT, UBA and WWE domain-containing protein 1/Upstream regulatory element-binding protein 1(URE-B1/URE-binding protein 1)/

Notes

For Research Use Only

UniProt ID	P51593
Antigen	HUWE1

Applications	ELISA
Applications Notes	Huwe1 ELISA Kit allows for the in vitro quantitative determination of Huwe1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10800046	ER0662	96T	$400		Add to Cart

Rat Huwe1 (E3 ubiquitin-protein ligase HUWE1) ELISA Kit

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