Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of HSP47 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP47 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-101
97
EDTA plasma(n=5)
87-105
92
heparin plasma(n=5)
85-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP47 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HSP47 ELISA Kit allows for the in vitro quantitative determination of HSP47 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat HSP47 (Heat Shock Protein 47) ELISA Kit