Loading ....
Contact us to order
Tel
+1 866.986.9598
Fine Test
view supplier detailsCredit card payments now incur a 3% fee.
Fine Test
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | ER0665 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | 46.875pg/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Range | 78.125-5000pg/ml | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Reactivity | Rat | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Duration | 4 hours | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Assay Type | Sandwich | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Description | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Hsd3b1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Hsd3b1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Hsd3b1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Alternative Names | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 ELISA Kit, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I ELISA Kit, 3-beta-HSD I ELISA Kit, 3-beta-hydroxy-5-ene steroid dehydrogenase ELISA Kit, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase ELISA Kit, 3-beta-hydroxysteroid 3-dehydrogenase ELISA Kit, Delta-5-3-ketosteroid isomerase ELISA Kit, Dihydrotestosterone oxidoreductase ELISA Kit, Steroid Delta-isomerase ELISA Kit, Trophoblast antigen FDO161G ELISA Kit, HSD3B1 ELISA Kit, 3BH ELISA Kit, HSDB3A ELISA Kit | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Detection Method | Double Antibody | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Kit Components |
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Recovery | Add a certain amount of Hsd3b1 into the sample. Calculate the recovery by comparing the measured value with the expected amount of Hsd3b1 in the sample.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Linearity | Dilute the sample with a certain amount of Hsd3b1 at 1:2, 1:4 and 1:8 to get the recovery range.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Specificity | Specifically binds with Hsd3b1 , no obvious cross reaction with other analogues. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Standard Curve | This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Components | Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Precision | Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Background | Hsd3b1/3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I(3-beta-HSD I)/ | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Stability | Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Notes | For Research Use Only | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Usage Notes | 
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE10801409 | ER0665 | 48T | $280 |
|
Add to Cart |
| BHE10801409 | ER0665 | 96T | $400 |
|
Add to Cart |
