Matrices listed below were spiked with certain level of HSD11B1 and the recovery rates were calculated by comparing the measured value to the expected amount of HSD11B1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-96
91
EDTA plasma(n=5)
88-101
96
heparin plasma(n=5)
86-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSD11B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HSD11B1 ELISA Kit allows for the in vitro quantitative determination of HSD11B1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat HSD11B1(Corticosteroid 11-beta-dehydrogenase isozyme 1) ELISA Kit