Rat homovanillic acid (HVA) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027785RA

Sensitivity

0.049 ng/mL

Detection Range

0.049 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

homovanillic acid (HVA)

Recovery

The recovery of dog GAST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog GAST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-102
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	HVA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Low-grade chronic inflammation induces behavioral stereotypy in rats. Erba.et al,Metab Brain Dis.,30(3):739-46 Agomelatine Protection in an LPS-Induced Psychosis-Relevant Behavior Model Inanir S. et al,Med Sci Monit,2015 21:3834-9. Levetiracetam attenuates rotenone-induced toxicity: A rat model of Parkinson's disease. Erba. et al,Environ Toxicol Pharmacol.,2016 42:226-30.

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