Rat Homocysteic acid,Hcy ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08896r

Sensitivity

0.195 nmol/mL

Detection Range

0.78 nmol/mL-50 nmol/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Homocysteic acid,Hcy

Recovery

The recovery of mouse SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	102	96-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-97
1:2	Average %	97
	Range %	92-102
1:4	Average %	88
	Range %	85-93
1:8	Average %	89
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Hcy

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Homocysteine alters osteoprotegerin/RANKL System in the osteoblast to promote bone loss: Pivotal role of redox regulator forkhead O1 Vijayan V et al,Free Radic Biol Med,2013 Mar 15;61C:72-84. Nitration-mediated deficiency of cystathionine B-synthase activity accelerates the progression of hyperhomocysteinemia Wang H.et al,Free Radic Biol Med,Nov 1

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