Matrices listed below were spiked with certain level of HO-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HO-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
99
EDTA plasma(n=5)
87-102
95
heparin plasma(n=5)
90-100
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HO-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
HO-1 ELISA Kit allows for the in vitro quantitative determination of HO-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.