Rat High mobility group protein B1,HMGB-1 ELISA Kit

Rat High mobility group protein B1,HMGB-1 ELISA Kit

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Mfr.No.

CSB-E08224r

Reactivity

Rat

Assay Duration

1-5h

Alternative Names

high-mobility group box 1, DKFZp686A04236, HMG1, HMG3, SBP-1, Amphoterin, OTTHUMP00000018196, OTTHUMP00000190860, OTTHUMP00000200117, Sulfoglucuronyl carbohydrate binding protein, high mobility group box 1, high mobility group prot

Recovery

The recovery of mouse BGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	100-108
EDTA plasma (n=4)	93	89-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	99-103
1:2	Average %	95
	Range %	91-99
1:4	Average %	88
	Range %	84-92
1:8	Average %	99
	Range %	95-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.378	2.496	2.437	2.319
20	1.863	1.965	1.914	1.796
10	1.432	1.467	1.450	1.332
5	1.025	1.090	1.058	0.940
2.5	0.666	0.644	0.655	0.537
1.25	0.437	0.470	0.454	0.336
0.625	0.269	0.288	0.279	0.161
0	0.116	0.119	0.118

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

UniProt ID	P63159

Applications	ELISA
Research Area	HMGB1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Inhibition of HMGB1 release via salvianolic acid B-mediated SIRT1 up-regulation protects rats against non-alcoholic fatty liver disease Zeng W. et al,Sci Rep,2015 3(5):16013. Baicalin inhibits toll-like receptor 2/4 expression and downstream signaling in rat experimental periodontitis Sun JY.et al,Int Immunopharmacol.,36:86-93 Fibroblast growth factor 2 protects against renal ischaemia/reperfusion injury by attenuating mitochondrial damage and proinflammatory signalling Tan XH.et al,J Cell Mol Med.,May 24 Coenzyme Q10 attenuates lung and liver fibrosis via modulation of autophagy in methotrexate treated rat Doaa I.Mohamed, et al,Biomedicine & Pharmacotherapy,November 5

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.