Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of HIF3A and the recovery rates were calculated by comparing the measured value to the expected amount of HIF3A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-101
95
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
86-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF3A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-99%
87-104%
92-104%
EDTA plasma(n=5)
85-101%
82-97%
87-101%
heparin plasma(n=5)
81-97%
84-98%
90-94%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9JHS2
Applications
ELISA
Applications Notes
HIF3A ELISA Kit allows for the in vitro quantitative determination of HIF3A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat HIF3A (Hypoxia-inducible factor 3-alpha) ELISA Kit