Rat hepatocyte growth factor,HGF ELISA kit

Rat hepatocyte growth factor,HGF ELISA kit

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Mfr.No.

CSB-E07346r

Alternative Names

hepatocyte growth factor (hepapoietin A; scatter factor), DFNB39, F-TCF, HGFB, HPTA, SF, OTTHUMP00000206710, fibroblast-derived tumor cytotoxic factor, hepatocyte growth factor, lung fibroblast-derived mitogen

Reactivity

Rat

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SAT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-92
EDTA plasma (n=4)	100	93-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SAT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-102
1:2	Average %	99
	Range %	92-102
1:4	Average %	89
	Range %	82-92
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.560	2.513	2.537	2.408
5	1.623	1.587	1.605	1.476
2.5	1.094	1.068	1.081	0.952
1.25	0.658	0.648	0.653	0.524
0.625	0.437	0.451	0.444	0.315
0.312	0.273	0.266	0.270	0.141
0.156	0.198	0.188	0.193	0.064
0	0.128	0.130	0.129

Species

Rattus norvegicus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P17945
Antigen	hepatocyte growth factor (hepapoietin A; scatter factor)

Applications

ELISA

Research Area

HGF

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508118	CSB-E07346r	96T	$615	Add to Cart
BHE10508118	CSB-E07346r	5x96T	$2952	Add to Cart
BHE10508118	CSB-E07346r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.