Rat hepatic lipase,HL ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E10032r

Alternative Names

lipase, hepatic, HDLCQ12, HL, HTGL, LIPH, lipase C

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-98
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-98
1:2	Average %	92
	Range %	81-105
1:4	Average %	94
	Range %	92-107
1:8	Average %	95
	Range %	86-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P07867
Antigen	Lipase, Hepatic

Applications

ELISA

Research Area

LIPC

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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