Rat heat shock protein,HSP47 ELISA Kit

Rat heat shock protein,HSP47 ELISA Kit

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Mfr.No.

CSB-E11328r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1), AsTP3, CBP1, CBP2, HSP47, PIG14, PPROM, RA-A47, SERPINH2, gp46, arsenic-transactivated protein 3, colligin-1, colligin-2, proliferation-inducing gene 14, rheumatoid arthritis antigen A-47, serine (or cys

Recovery

The recovery of human sCD86 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-95
EDTA plasma (n=4)	103	100-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD86 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	91-95
1:2	Average %	96
	Range %	91-101
1:4	Average %	99
	Range %	92-106
1:8	Average %	86
	Range %	82-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.500	2.659	2.580	2.425
10	2.167	2.035	2.101	1.946
5	1.423	1.544	1.484	1.329
2.5	0.888	0.852	0.870	0.715
1.25	0.622	0.631	0.627	0.472
0.625	0.414	0.437	0.426	0.271
0.312	0.268	0.281	0.275	0.120
0	0.149	0.161	0.155

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29457

Applications	ELISA
Research Area	SERPINH1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508103	CSB-E11328r	96T	$790	Add to Cart
BHE10508103	CSB-E11328r	5x96T	$3792	Add to Cart
BHE10508103	CSB-E11328r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.