Rat heat shock protein 27,HSP-27 ELISA Kit

Rat heat shock protein 27,HSP-27 ELISA Kit

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Mfr.No.

CSB-E09240r

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

heat shock 27kDa protein 1, CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, estrogen-regulated 24 kDa protein, heat shock 27kD protein 1, heat shock protein beta-1, stress-responsive protein 27

Recovery

The recovery of human thymosin ?4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-94
EDTA plasma (n=4)	102	96-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human thymosin ?4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-96
1:2	Average %	98
	Range %	92-105
1:4	Average %	94
	Range %	90-102
1:8	Average %	97
	Range %	92-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
5000	2.411	2.312	2.362	2.206
2500	1.835	1.736	1.786	1.630
1250	1.212	1.203	1.208	1.052
625	0.703	0.694	0.699	0.543
312	0.394	0.404	0.399	0.243
156	0.277	0.286	0.282	0.126
78	0.223	0.228	0.226	0.070
0	0.155	0.156	0.156	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P42930

Applications	ELISA
Research Area	HSPB1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508097	CSB-E09240r	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.