Rat heart fatty acid binding protein(h-FABP)ELISA Kit

Rat heart fatty acid binding protein(h-FABP)ELISA Kit

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Mfr.No.

CSB-E16184r

Alternative Names

fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor), FABP11, H-FABP, MDGI, O-FABP, Fatty acid-binding protein 3, muscle, fatty acid binding protein 11, fatty acid binding protein 3, mammary-derived growth inhibitor

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse MT-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	101-106
EDTA plasma (n=4)	95	92-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MT-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	102
	Range %	99-105
1:4	Average %	89
	Range %	86-93
1:8	Average %	94
	Range %	92-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	1.975	2.022	1.999	1.842
500	1.362	1.377	1.370	1.213
250	0.811	0.862	0.837	0.680
125	0.433	0.439	0.436	0.279
62.5	0.304	0.330	0.317	0.160
31.2	0.262	0.274	0.268	0.111
15.6	0.214	0.220	0.217	0.060
0	0.163	0.150	0.157

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P07483
Antigen	fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)

Applications

ELISA

Research Area

FABP3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508095	CSB-E16184r	96T	$695	Add to Cart
BHE10508095	CSB-E16184r	5x96T	$3336	Add to Cart
BHE10508095	CSB-E16184r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.