HDL-C ELISA Kit, high density lipoprotein cholesterol ELISA Kit
Reactivity
Rat
Detection Method
Double Antibody
Detection Range
0.391-25ng/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of HDL-C and the recovery rates were calculated by comparing the measured value to the expected amount of HDL-C in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
93
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
90-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HDL-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
87-101%
92-104%
EDTA plasma(n=5)
82-95%
86-101%
90-99%
heparin plasma(n=5)
83-99%
82-98%
82-100%
Sensitivity
< 0.234ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
HDL-CELISA Kit allows for the in vitro quantitative determination of HDL-C concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat HDL-C (high density lipoprotein cholesterol) ELISA Kit