Rat HBEGF(Heparin Binding Epidermal Growth Factor Like Growth Factor) ELISA Kit

Mfr.No.

ELK2766

Alternative Names

HB-EGF; DTR; DTS; DTSF; HEGFL; Diphtheria Toxin Receptor; Proheparin-binding EGF-like growth factor; Diphtheria toxin receptor

Reactivity

Rat

Detection Range

15.63-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant HBEGF and the recovery rates were calculated by comparing the measured value to the expected amount of HBEGF in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	88-103%	95%
Heparin plasma(n=5)	92-106%	99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HBEGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	82-94%	85-96%	78-90%	91-99%
EDTA plasma(n=5)	89-97%	86-102%	94-106%	85-97%
Heparin plasma(n=5)	87-99%	85-96%	87-101%	89-102%

Typical Data

<table> <tbody><tr> <th>Concentration (pg/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>1000.00</td> <td>2.218</td> <td>2.133</td> </tr> <tr> <td>500.00</td> <td>1.621</td> <td>1.536</td> </tr> <tr> <td>250.00</td> <td>1.088</td> <td>1.003</td> </tr> <tr> <td>125.00</td> <td>0.838</td> <td>0.753</td> </tr> <tr> <td>62.50</td> <td>0.601</td> <td>0.516</td> </tr> <tr> <td>31.25</td> <td>0.389</td> <td>0.304</td> </tr> <tr> <td>15.63</td> <td>0.205</td> <td>0.120</td> </tr> <tr> <td>0.00</td> <td>0.085</td> <td>0.000</td> </tr> </tbody></table>

Sensitivity

6.1 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	Q06175
Antigen	HBEGF
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HBEGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HBEGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HBEGF in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;
Protocol	<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK2766-1.pdf" target="_blank">Datasheet</a>
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205380	ELK2766	48T	$320		Add to Cart
BHE15205380	ELK2766	96T	$458		Add to Cart

Rat HBEGF(Heparin Binding Epidermal Growth Factor Like Growth Factor) ELISA Kit

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