Rat Haptoglobin,Hpt/HP ELISA KIT

Rat Haptoglobin,Hpt/HP ELISA KIT

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Mfr.No.

CSB-E08587r

Sensitivity

0.039 ug/mL

Detection Range

0.156 ug/mL-10 ug/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

haptoglobin, BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide

Recovery

The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-110
EDTA plasma (n=4)	99	90-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-109
1:4	Average %	105
	Range %	92-114
1:8	Average %	95
	Range %	82-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.667	2.684	2.676	2.525
250	1.934	1.939	1.937	1.786
125	1.123	1.119	1.121	0.970
62.5	0.690	0.674	0.682	0.531
31.2	0.462	0.471	0.467	0.316
15.6	0.316	0.306	0.311	0.160
7.8	0.236	0.225	0.231	0.080
0	0.152	0.150	0.151	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P06866

Applications	ELISA
Research Area	HP

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508094	CSB-E08587r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.