Matrices listed below were spiked with certain level of H3cit-NE and the recovery rates were calculated by comparing the measured value to the expected amount of H3cit-NE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
92
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
89-95
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of H3cit-NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-102%
85-103%
88-102%
EDTA plasma(n=5)
91-101%
85-96%
86-100%
heparin plasma(n=5)
86-99%
86-96%
85-92%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
H3cit-NE ELISA kit allows for the in vitro quantitative determination of H3cit-NE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat H3cit-NE(neutrophil extracellular trap) ELISA kit