Rat guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA kit

Mfr.No.

CSB-EL010048RA

Alternative Names

guanylate cyclase activator 2B (uroguanylin), GCAP-II, UGN, guanylate cyclase activator 2B, uroguanylin

Reactivity

Rat

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human MICB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MICB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	99
	Range %	93-104
1:4	Average %	95
	Range %	89-99
1:8	Average %	94
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	P70668
Antigen	guanylate cyclase activator 2B (uroguanylin)

Applications

ELISA

Research Area

GUCA2B

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508091	CSB-EL010048RA	10x96T	$6602.50	Add to Cart