Matrices listed below were spiked with certain level of GS and the recovery rates were calculated by comparing the measured value to the expected amount of GS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
97
EDTA plasma(n=5)
87-103
97
heparin plasma(n=5)
85-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.