Rat growth hormone,GH ELISA KIT

Mfr.No.

CSB-E07342r

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

growth hormone 1, GH, GH-N, GHN, IGHD1B, hGH-N, pituitary growth hormone

Recovery

The recovery of mouse TIMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-100
EDTA plasma (n=4)	101	95-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TIMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-104
1:2	Average %	94
	Range %	88-98
1:4	Average %	98
	Range %	91-102
1:8	Average %	97
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P09916

Applications	ELISA
Research Area	GH1

Storage	Store at 2-8°C. Please refer to protocol.

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