Rat Growth Differentiation Factor 8

Mfr.No.

E1999Ra

Sensitivity

0.146ng/mL

Detection Range

0.3-90ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

GDF-8;Growth/differentiation factor 8;MSTN;Myostatin

Molecular Weight

42829

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1999Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	29.19	0.99	3.38
Intra-Assay	2	18	35.94	0.85	2.38
Intra-Assay	3	18	18.84	0.93	4.93

Background

Myostatin (also known as growth differentiation factor 8, abbreviated GDF8) is a myokine, a protein produced and released by myocytes that acts on muscle cells to inhibit muscle cell growth. In humans it is encoded by the MSTN gene. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family.

Protocol

These standard curves of E1999Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	1.941	2.003	1.951
	2.064
24ng/mL	0.903	0.961	0.91
	1.02
12ng/mL	0.553	0.509	0.458
	0.466
6ng/mL	0.206	0.246	0.195
	0.287
3ng/mL	0.11	0.139	0.087
	0.168
0ng/mL	0.062	0.052	0
	0.042

Gene ID	29152
UniProt ID	O35312

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113198	E1999Ra	48T	$320		Add to Cart
BHE12113198	E1999Ra	96T	$458		Add to Cart

Rat Growth Differentiation Factor 8 - MSTN ELISA Kit

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