Matrices listed below were spiked with certain level of GPX4 and the recovery rates were calculated by comparing the measured value to the expected amount of GPX4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
95
EDTA plasma(n=5)
95-101
97
heparin plasma(n=5)
89-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPX4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
88-105%
89-103%
EDTA plasma(n=5)
97-101%
90-101%
93-99%
heparin plasma(n=5)
95-100%
94-99%
95-100%
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Notes
For Research Use Only
UniProt ID
P36970
Applications
ELISA
Applications Notes
GPX4ELISA Kit allows for the in vitro quantitative determination of GPX4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Storage
2-8 °C for 6 months
Reviews of Rat GPX4 (Phospholipid hydroperoxide glutathione peroxidase) ELISA Kit