Rat gonadotropin-releasing hormone,GnRH ELISA Kit

Mfr.No.

CSB-E08037r

Sensitivity

1 pg/mL

Detection Range

2 pg/mL-150 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

gonadotropin-releasing hormone 1 (luteinizing-releasing hormone), GNRH, GRH, LHRH, LNRH, gonadotropin-releasing hormone 1, gonadotropin-releasing hormone 1 (leutinizing-releasing hormone), gonadotropin-releasing hormone 1 (lutienizing hormone-releasing hormone)

Recovery

The recovery of mouse AMBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	97	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse AMBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	104
	Range %	101-107
1:200	Average %	96
	Range %	93-99
1:400	Average %	94
	Range %	90-97
1:800	Average %	91
	Range %	84-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
100	0.140	0.145	0.143
50	0.357	0.366	0.362
25	0.569	0.578	0.574
12.5	0.865	0.898	0.882
6.25	1.544	1.532	1.538
3.125	2.083	2.098	2.091
1.563	2.469	2.466	2.468
0	2.844	2.878	2.861

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P07490

Applications	ELISA
Research Area	GNRH1

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Withania somnifera aqueous extract facilitates the expression and release of GnRH: in vitro and in vivo study Kataria H. et al,Neurochem Int,2015 Aug 6.pii: S0197-0186(15)30029-2. Effects of chronic unpredictable mild stress on ovarian reserve in female rats: Feasibility analysis of a rat model of premature ovarian failure Xiao‑Yan Fu.et al,Molecular Medicine Reports,May 8 Impact of Sleep Deprivation on the Hypothalamic-Pituitary-Gonadal Axis and Erectile Tissue Lee DS, et al,Journal of Sexual Medicine,January 7

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SKU	Supplier No.	Size	Your price
BHE10508070	CSB-E08037r	96T	$856	Add to Cart
BHE10508070	CSB-E08037r	5x96T	$4108.80	Add to Cart
BHE10508070	CSB-E08037r	10x96T	$8132	Add to Cart

Rat gonadotropin-releasing hormone,GnRH ELISA Kit

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