Rat Glycine N-methyltransferase

Mfr.No.

E1991Ra

Sensitivity

9.85ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Folate-binding protein;Glycine N-methyltransferase;GNMT

Molecular Weight

32549

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1991Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1329.1	38.24	2.9
Intra-Assay	2	18	772.6	40.24	5.2
Intra-Assay	3	18	323.6	16.5	5.1

Background

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Protocol

These standard curves of E1991Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.237	2.33	2.288
	2.422
1200ng/L	1.383	1.284	1.243
	1.185
600ng/L	0.645	0.676	0.634
	0.706
300ng/L	0.325	0.322	0.28
	0.318
150ng/L	0.21	0.171	0.129
	0.132
0ng/L	0.052	0.041	0
	0.031

Gene ID	25134
UniProt ID	P13255

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113190	E1991Ra	48T	$320		Add to Cart
BHE12113190	E1991Ra	96T	$458		Add to Cart

Rat Glycine N-methyltransferase - GNMT ELISA Kit

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