Rat Glycated hemoglobin A1c,GHbA1c ELISA Kit

Rat Glycated hemoglobin A1c,GHbA1c ELISA Kit

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Mfr.No.

CSB-E08140r

Alternative Names

Glycated hemoglobin A1c,GHbA1c

Reactivity

Rat

Detection Range

0.019 ng/mL-80 ng/mL

Sample Type

Lysate for rbc

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-104
EDTA plasma (n=4)	95	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	96
	Range %	88-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.012	1.983	1.998	1.952
1000	1.338	1.341	1.340	1.294
500	0.775	0.828	0.802	0.756
167	0.357	0.349	0.353	0.307
84	0.224	0.235	0.230	0.184
0	0.047	0.045	0.046	?

Species

Rattus norvegicus

Sensitivity

0.019 ng/mL

Limit Of Detection

450 nm

Antigen	Glycated hemoglobin A1c,GHbA1c

Applications

ELISA

Research Area

GHbA1c

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dysfunction of methionine sulfoxide reductases to repair damaged proteins by nickel nanoparticles Po-Hao Feng. et al,Chem Biol Interact,2015 May 13;236:82-89. Low-dose Insulin Treatment Ameliorate Glucose Metabolism in Type 1Diabetic Rats Athena Xin Hui Ng.et al,Diabetes & Metabolism,2016 ,7(1):8 Pharmacotherapy with Thymoquinone Improved Pancreatic B-Cell Integrity and Functional Activity, Enhanced Islets Revascularization, and Alleviated Metabolic and Hepato-Renal Disturbances in Streptozotocin-Induced Diabetes in Rats El-Shemi AG.et al,Pharmacology,Sep 20 Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Passant E. Moustafa.et al,Inflammation,27 April

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SKU	Supplier No.	Size	Your price
BHE10508056	CSB-E08140r	96T	$856	Add to Cart
BHE10508056	CSB-E08140r	5x96T	$4108.80	Add to Cart
BHE10508056	CSB-E08140r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.