Rat Glycated hemoglobin A1c,GHbA1c ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<10%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08140r

Sensitivity

0.019 ng/mL

Detection Range

0.019 ng/mL-80 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Glycated hemoglobin A1c,GHbA1c

Recovery

The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-104
EDTA plasma (n=4)	95	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-98
1:2	Average %	96
	Range %	88-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	GHbA1c

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Dysfunction of methionine sulfoxide reductases to repair damaged proteins by nickel nanoparticles Po-Hao Feng. et al,Chem Biol Interact,2015 May 13;236:82-89. Low-dose Insulin Treatment Ameliorate Glucose Metabolism in Type 1Diabetic Rats Athena Xin Hui Ng.et al,Diabetes & Metabolism,2016 ,7(1):8 Pharmacotherapy with Thymoquinone Improved Pancreatic B-Cell Integrity and Functional Activity, Enhanced Islets Revascularization, and Alleviated Metabolic and Hepato-Renal Disturbances in Streptozotocin-Induced Diabetes in Rats El-Shemi AG.et al,Pharmacology,Sep 20 Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Passant E. Moustafa.et al,Inflammation,27 April

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