Rat Glutathione Synthetase

Mfr.No.

E1989Ra

Sensitivity

0.024ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Glutathione Synthase;Glutathione synthetase;GSH synthetase;GSH-S;GSS

Molecular Weight

52345

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1989Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.25	0.34	4.14
Intra-Assay	2	18	3.27	0.19	5.75
Intra-Assay	3	18	1.15	0.05	4.23

Background

Glutathione synthetase (GSS) (EC 6.3.2.3) is the second enzyme in the glutathione (GSH) biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Glutathione synthetase is also a potent antioxidant. It is found in many species including bacteria, yeast, mammals, and plants.

Protocol

These standard curves of E1989Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.146	2.161	2.107
	2.176
6ng/mL	1.391	1.445	1.391
	1.499
3ng/mL	0.815	0.811	0.758
	0.807
1.5ng/mL	0.532	0.482	0.428
	0.431
0.75ng/mL	0.285	0.251	0.197
	0.216
0ng/mL	0.054	0.054	0
	0.053

Gene ID	25458
UniProt ID	P46413

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113188	E1989Ra	48T	$320		Add to Cart
BHE12113188	E1989Ra	96T	$458		Add to Cart

Rat Glutathione Synthetase - GSS ELISA Kit

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