Rat Glucose-6-phosphsatase

Mfr.No.

E1611Ra

Sensitivity

0.093ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Glucose-6-phosphsatase (also known as G6PC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

G6Pase;G-6-Pase;G6PC;Glucose-6-phosphatase

Molecular Weight

40556

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1611Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7.1	0.47	6.6
Intra-Assay	2	18	26.2	1	3.8
Intra-Assay	3	18	11.2	0.51	4.6

Background

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

Protocol

These standard curves of E1611Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
36ng/ml	2.164	2.268	2.234
	2.371
18ng/ml	1.342	1.299	1.265
	1.256
9ng/ml	0.721	0.779	0.745
	0.838
4.5ng/ml	0.498	0.439	0.405
	0.38
2.25ng/ml	0.188	0.19	0.156
	0.192
0ng/ml	0.033	0.034	0
	0.035

Gene ID	25634
UniProt ID	P43428

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112818	E1611Ra	48T	$320		Add to Cart
BHE12112818	E1611Ra	96T	$458		Add to Cart

Rat Glucose-6-phosphsatase - G6PC ELISA Kit

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