Rat glucagon-like peptide-1,GLP-1 ELISA Kit

Rat glucagon-like peptide-1,GLP-1 ELISA Kit

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Mfr.No.

CSB-E08117r

Alternative Names

glucagon-like peptide-1,GLP-1

Reactivity

Rat

Detection Range

0.625 pg/mL-40 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	102	95-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-95
1:2	Average %	97
	Range %	92-101
1:4	Average %	106
	Range %	98-113
1:8	Average %	94
	Range %	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
10	2.645	2.566	2.606	2.421
5	2.015	1.855	1.935	1.750
2.5	1.194	1.155	1.175	0.990
1.25	0.777	0.796	0.787	0.602
0.625	0.492	0.461	0.477	0.292
0.312	0.335	0.344	0.340	0.155
0.156	0.278	0.288	0.283	0.098
0	0.191	0.178	0.185	?

Species

Rattus norvegicus

Sensitivity

0.156 pg/mL

Limit Of Detection

450 nm

Antigen	glucagon-like peptide-1,GLP-1

Applications

ELISA

Research Area

Glp1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Inhibiting Roles of Berberine in Gut Movement of Rodents are Related to Activation of the Endogenous Opioid System Feng Y et al,Phytother Res,2013 Jan 21. Changes in ghrelin, CCK, GLP-1, and peroxisome proliferator-activated receptors in a hypoxia-induced anorexia rat model Duraisamy AJ. et al,Endokrynol Pol,2015 66(4):334-41. Sitagliptin inhibits endothelin-1 expression in the aortic endothelium of rats with streptozotocin-induced diabetes by suppressing the nuclear factor-kB/IkBa system through the activation of AMP-activated protein kinase Tang S T.et al,International journal of molecular medicine,37(6): 1558-1566 Influence of Dipeptidyl Peptidase-IV Inhibitor Sitagliptin on Extracellular Signal-Regulated Kinases 1/2 Signaling in Rats with Diabetic Nephropathy Ren X.et al,Pharmacology,Mar 23;100(1-2):1-13. Duodenum Exclusion Alone Is Sufficient to Improve Glucose Metabolism in STZ-Induced Diabetes Rats Weihang W.et al,Obesity Surgery,May 22

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SKU	Supplier No.	Size	Your price
BHE10508033	CSB-E08117r	96T	$856	Add to Cart
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BHE10508033	CSB-E08117r	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.