Rat glial cell line-derived neurotrophic factor,GDNF ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04566r

Alternative Names

glial cell derived neurotrophic factor, ATF1, ATF2, HFB1-GDNF, astrocyte-derived trophic factor, glial cell line derived neurotrophic factor

Reactivity

Rat

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-99
1:10	Average %	97
	Range %	93-105
1:20	Average %	85
	Range %	82-89
1:40	Average %	90
	Range %	85-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q07731
Antigen	glial cell derived neurotrophic factor

Applications

ELISA

Research Area

GDNF

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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