Rat ghrelin (GHRL) ELISA kit

Mfr.No.

CSB-E09816r

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ghrelin/obestatin prepropeptide, MTLRP, obestatin, OTTHUMP00000207794, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin, ghrelin/obestatin preprohormone, m

Recovery

The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	91
	Range %	89-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.184	2.201	2.193	2.099
500	1.628	1.643	1.636	1.542
250	1.023	1.015	1.019	0.925
125	0.565	0.575	0.570	0.476
62.5	0.318	0.305	0.312	0.218
31.2	0.179	0.192	0.186	0.092
15.6	0.129	0.133	0.131	0.037
0	0.095	0.092	0.094	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9QYH7

Applications	ELISA
Research Area	GHRL

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10508027	CSB-E09816r	96T	$695	Add to Cart
BHE10508027	CSB-E09816r	5x96T	$3336	Add to Cart
BHE10508027	CSB-E09816r	10x96T	$6602.50	Add to Cart

Rat ghrelin (GHRL) ELISA kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat ghrelin (GHRL) ELISA kit

Rate it now..!!