Rat ghrelin (GHRL) ELISA kit

Mfr.No.

CSB-E09816r

Alternative Names

ghrelin/obestatin prepropeptide, MTLRP, obestatin, OTTHUMP00000207794, OTTHUMP00000207795, OTTHUMP00000207796, OTTHUMP00000207798, ghrelin, growth hormone secretagogue receptor ligand, ghrelin/obestatin, ghrelin/obestatin preprohormone, m

Reactivity

Rat

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	91
	Range %	89-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.184	2.201	2.193	2.099
500	1.628	1.643	1.636	1.542
250	1.023	1.015	1.019	0.925
125	0.565	0.575	0.570	0.476
62.5	0.318	0.305	0.312	0.218
31.2	0.179	0.192	0.186	0.092
15.6	0.129	0.133	0.131	0.037
0	0.095	0.092	0.094	?

Species

Rattus norvegicus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9QYH7
Antigen	ghrelin/obestatin prepropeptide

Applications

ELISA

Research Area

GHRL

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508027	CSB-E09816r	96T	$695	Add to Cart
BHE10508027	CSB-E09816r	5x96T	$3336	Add to Cart
BHE10508027	CSB-E09816r	10x96T	$6602.50	Add to Cart

Rat ghrelin (GHRL) ELISA kit

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