Rat GDF5/Bmp 14 ELISA Kit PicoKine®

Mfr.No.

EK1504

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.082	0.144	0.173	0.283	0.409	0.787	1.385	2.050

Description

Rat GDF5/Bmp 14 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gdf5 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth and differentiation factor-5 ;Gdf5 ;gdf-5 ;

Molecular Weight

7491 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A376-R495

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat GDF5

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	60	323	894	59	339	908
Standard deviation	3.84	14.53	50.06	4.72	21.01	57.2
CV (%)	6.4%	4.5%	5.6%	8%	6.2%	6.3%

Background

Growth/differentiation factor 5 is a protein that in humans is encoded by the GDF5 gene. GDF5 is a protein belonging to the transforming growth factor beta superfamily that is expressed in the developing central nervous system, and has a role in skeletal and joint development. It also increases the survival of neurones that respond to the neurotransmitter dopamine, and is a potential therapeutic molecule associated with Parkinson's disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	60	59	68	62	62	3.49	5.6%
Sample 2	323	317	316	281	309	16.52	5.3%
Sample 3	894	859	910	831	873	30.69	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	252835
Gene Name	Gdf5
UniProt ID	Q8K4X5
Protein Name	Growth and differentiation factor-5

Applications	ELISA
Research Area	Metabolism,Mitochondrial,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001093	EK1504	96 wells/kit	$750		Add to Cart

Rat GDF5/Bmp 14 ELISA Kit PicoKine®

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