Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of GCLM and the recovery rates were calculated by comparing the measured value to the expected amount of GCLM in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
87-103
97
heparin plasma(n=5)
86-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCLM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GCLM ELISA Kit allows for the in vitro quantitative determination of GCLM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit