Rat gastric inhibitory polypeptide (Gip) ELISA kit

Rat gastric inhibitory polypeptide (Gip) ELISA kit

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Mfr.No.

CSB-E17969r

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

gastric inhibitory polypeptide (Gip), glucose-dependent insulinotropic polypeptide, gastric inhibitory polypeptide (Gip), glucose-dependent insulinotropic polypeptide

Recovery

The recovery of human DHH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-96
EDTA plasma (n=4)	96	94-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DHH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	89
	Range %	84-92
1:10	Average %	99
	Range %	95-101
1:20	Average %	99
	Range %	95-102
1:40	Average %	95
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.418	2.402	2.410	2.297
500	1.766	1.880	1.823	1.710
250	1.223	1.213	1.218	1.105
125	0.689	0.707	0.698	0.585
62.5	0.453	0.432	0.443	0.330
31.2	0.324	0.331	0.328	0.215
15.6	0.224	0.218	0.221	0.108
0	0.115	0.111	0.113	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q06145

Applications	ELISA
Research Area	Gip

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10508017	CSB-E17969r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.