Rat GAS6 ELISA Kit PicoKine®

Mfr.No.

EK1915

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.022	0.056	0.115	0.186	0.336	0.598	1.116	1.898

Description

Rat GAS6 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gas6 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth arrest-specific protein 6; GAS-6; AXL receptor tyrosine kinase ligand; Growth-potentiating factor; GPF; Gas6

Immunogen

Expression system for standard: NS0; Immunogen sequence: D115-P673

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat GAS6

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	73	308	856	75	321	969
Standard deviation	3.94	13.24	48.79	4.65	20.22	73.64
CV (%)	5.4%	4.3%	5.7%	6.2%	6.3%	7.6%

Protein Function

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Background

GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	73	66	71	70	70	2.54	3.6%
Sample 2	308	302	310	268	297	17.00	5.7%
Sample 3	856	806	872	876	852	27.87	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2621
Gene Name	Gas6
UniProt ID	Q63772
Tissue Specificity	Plasma.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Hematopoietic Progenitors,Mesenchymal Stem Cells,Myeloid,Protein Phosphorylation,Receptor Tyrosine Kinases,Signal Transduction,Stem Cells,Tyrosine Kinases

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001424	EK1915	96 wells/kit	$499		Add to Cart

Rat GAS6 ELISA Kit PicoKine®

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