Rat Galectin-3-binding protein(LGALS3BP) ELISA kit

Rat Galectin-3-binding protein(LGALS3BP) ELISA kit

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Mfr.No.

CSB-EL012888RA

Detection Range

0.78 ug/mL-50 ug/mL

Sensitivity

0.195 ug/mL

Reactivity

Rat

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lectin, galactoside-binding, soluble, 3 binding protein, 90K, BTBD17B, MAC-2-BP, L3 antigen, Mac-2-binding protein, galectin 3 binding protein, serum protein 90K

Recovery

The recovery of human TIH3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TIH3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	92
	Range %	86-99
1:40	Average %	97
	Range %	92-103
1:80	Average %	86
	Range %	80-89
1:160	Average %	90
	Range %	83-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
5000	0.083	0.085	0.084
2500	0.139	0.147	0.143
1250	0.223	0.220	0.222
625	0.329	0.347	0.338
312.5	0.572	0.614	0.593
156.3	0.814	0.849	0.832
78.13	1.228	1.259	1.244
0	2.081	2.226	2.154

Species

Rattus norvegicus

Limit Of Detection

450 nm

UniProt ID	O70513
Antigen	lectin, galactoside-binding, soluble, 3 binding protein

Applications

ELISA

Research Area

LGALS3BP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508009	CSB-EL012888RA	96T	$695	Add to Cart
BHE10508009	CSB-EL012888RA	5x96T	$3336	Add to Cart
BHE10508009	CSB-EL012888RA	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.