Matrices listed below were spiked with certain level of GAGs and the recovery rates were calculated by comparing the measured value to the expected amount of GAGs in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
96
EDTA plasma(n=5)
85-99
92
heparin plasma(n=5)
86-98
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAGs and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
GAGs ELISA Kit allows for the in vitro quantitative determination of GAGs concentrations in serum, plasma, tissue homogenates and other biological fluids.