Matrices listed below were spiked with certain level of G-6-Pase/G6PT/GSD and the recovery rates were calculated by comparing the measured value to the expected amount of G-6-Pase/G6PT/GSD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
96
EDTA plasma(n=5)
86-104
96
heparin plasma(n=5)
87-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of G-6-Pase/G6PT/GSD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
G-6-Pase ELISA Kit allows for the in vitro quantitative determination of G-6-Pase/G6PT/GSD concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat G-6-Pase(Glucose-6-phosphatase) ELISA Kit