Rat Fractalkine,FK ELISA Kit

Mfr.No.

CSB-E04560r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-X3-C motif) ligand 1, ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin, small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin), small inducible cytokine subfamily D (

Recovery

The recovery of human TrpV1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TrpV1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

84-98

1:2

Average %

Range %

89-103

1:4

Average %

Range %

87-96

1:8

Average %

Range %

80-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.987	2.149	2.068	1.906
10	1.633	1.573	1.603	1.441
5	1.119	1.137	1.128	0.966
2.5	0.774	0.790	0.782	0.620
1.25	0.441	0.461	0.451	0.289
0.625	0.324	0.306	0.315	0.153
0.312	0.229	0.231	0.230	0.068
0	0.161	0.162	0.162	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	O55145

Applications	ELISA
Research Area	CX3CL1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507995	CSB-E04560r	96T	$595	Add to Cart
BHE10507995	CSB-E04560r	5x96T	$2856	Add to Cart
BHE10507995	CSB-E04560r	10x96T	$5652.50	Add to Cart

Rat Fractalkine,FK ELISA Kit

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