Matrices listed below were spiked with certain level of FOXO3 and the recovery rates were calculated by comparing the measured value to the expected amount of FOXO3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-105
98
EDTA plasma(n=5)
93-102
96
heparin plasma(n=5)
90-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FOXO3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-98%
98-104%
90-102%
EDTA plasma(n=5)
91-96%
93-100%
94-98%
heparin plasma(n=5)
94-100%
94-99%
94-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
D3ZBQ1
Applications
ELISA
Applications Notes
FOXO3 ELISA Kit allows for the in vitro quantitative determination of FOXO3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat FOXO3(Forkhead box protein O3) ELISA Kit