Matrices listed below were spiked with certain level of Foxo1 and the recovery rates were calculated by comparing the measured value to the expected amount of Foxo1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-105
98
EDTA plasma(n=5)
97-102
99
heparin plasma(n=5)
95-103
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Foxo1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
95-104%
92-102%
98-105%
EDTA plasma(n=5)
89-101%
99-101%
90-98%
heparin plasma(n=5)
91-100%
95-99%
90-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
G3V7R4
Applications
ELISA
Applications Notes
Foxo1 ELISA Kit allows for the in vitro quantitative determination of Foxo1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Foxo1(Forkhead box protein O1) ELISA Kit