rat follicle-stimulating hormone,FSH ELISA Kit

rat follicle-stimulating hormone,FSH ELISA Kit

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Mfr.No.

CSB-E06869r

Reactivity

Rat

Detection Range

0.156 ng/ml - 10ng/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse S100A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse S100A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-103
1:200	Average %	85
	Range %	80-90
1:400	Average %	103
	Range %	99-108
1:800	Average %	98
	Range %	94-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.418	2.524	2.471	2.277
2000	1.854	1.917	1.886	1.692
1000	1.360	1.294	1.327	1.133
500	0.912	0.893	0.903	0.709
250	0.659	0.627	0.643	0.449
125	0.401	0.421	0.411	0.217
62.5	0.312	0.297	0.305	0.111
0	0.197	0.190	0.194

Species

Rattus norvegicus

Sensitivity

0.039 ng/ml

Limit Of Detection

450 nm

UniProt ID	P18427
Antigen	follicle-stimulating hormone,FSH

Applications

ELISA

Research Area

FSH

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Chronic Administration of High Doses of NandroloneDecanoate on the Pituitary-Gonadal Axis in Male Rats Alsi J. et al,Basic Clin Pharmacol Toxicol,2009 Nov;105(5):307-14. Alterations in gene expression and steroidogenesis in the testes of transient cerebral ischemia in male rats Zhao BH et al,Chinese Medical Journal,2012 Jun;125(12):2168-72 Evaluation of effects of repeated sevoflurane exposure on rat testicular tissue and reproductive hormones Kaya Z et al,Inhal Toxicol.,2013 Mar;25(4):192-8. Hormonal and histological changes in the ovaries with high-doses of methylprednisolone administration for acute spinal cord injury: An experimental study Osmanaaolu MA et al,J Obstet Gynaecol,2013 Aug;33(6):585-90. Impact of 900 MHz electromagnetic field exposure on main male reproductive hormone levels: a Rattus norvegicus model Sepehrimanesh M et al,Int J Biometeorol,2013 Dec 20. Effects of the phytoestrogen genistein on the development of the reproductive system of Sprague Dawley rats Siti Rosmani Md Zin et al,Clinics,2013 68(2):253-62. A Chinese herbal formula, Wuzi Yanzong Pill (), improves spermatogenesis by modulating the secretory function of Sertoli cells Xu YP et al,Chin J Integr Med,2014 Mar;20(3):194-9. A Survey of the Relationship Between Noised Pollution, Honey and Vitamin E and Plasma Level of Blood Sexual Hormones in Noise-Exposed Rats Asghar Rajabzadeh et al,Jentashapir Journal of Health Research,2015 Feb;6(1): e27331 Effect of Excess Iodine on Oxidative StressMarkers, Steroidogenic-Enzyme Activities, Testicular Morphology, and Functions in Adult Male Rats Chakraborty A. et al,Biol Trace Elem Res,2015 Dec 23. Effect of honey on the reproductive system of male rat offspring exposed to prenatal restraint stress Haron MN. et al,Andrologia,2015 Aug 20. Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats. Khalili L.et al,EXCLI J.,14:75-82 Toxicological evaluation of isopropylparaben and isobutylparaben mixture in Sprague-Dawley rats following 28 days of dermal exposure Kim MJ. et al,Regul Toxicol Pharmacol,2015 Sep 7.pii: S0273-2300(15)30045-3. Honey and Vitamin E Restore the Plasma Level of GonadalHormones and Improve the FertilizationCapacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015 2(2):64-68 The Effect of Intraventricular Administration of Zinc on Serum LH, FSH, Prolactin, and Testosterone in Male Rats Shahraki M R.et al,Zahedan Journal of Research in Medical Sciences,2015 17(9) Mammary gland epithelial changes in thyroidectomized female rats Vasilios Pergialiotis et al,JBUON,2015 Jan-Feb;20(1):57-61 Increase in endogenous estradiol in the progeny of obese rats is associated with precocious puberty and altered follicular development in adulthood Ambrosetti V. et al,Endocrine,2016 Jan 14. Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis Han X F.et al,Asian journal of andrology,,18(3): 485. Acetylshikonin from Zicao exerts antifertility effects at high dose in rats by suppressing the secretion of GTH. He Y.et al,Biochem Biophys Res Commun.,/ Sertraline-induced reproductive toxicity in male rats: evaluation of possible underlying mechanisms Ozlem Atli.et al,ajandrology,Dec 13 Protective Antioxidant Efficiency of Garlic against Lead-Induced Renal and Testicular Toxicity in Adult Male Rats Salem NA.et al,Heavy Metal Toxicity and Diseases,January High-Cholesterol Diet Disrupts the Levels of Hormones Derived from Anterior Pituitary Basophilic Cells Yang J.et al,J Neuroendocrinol.,2016 ,28(3). Effect of Intravenous Administration of Cobalt Chloride to Horses on Clinical and Hemodynamic Variables Burns TA.et al,J Vet Intern Med,Dec 29 Effect of amifostine on sperm DNA fragmentation and testes after radioiodine treatment Cigdem Cebi Sen.et al,Journal of Veterinary Research,Dec 27 Kisspeptin level in the aging ovary is regulated by the sympathetic nervous system D Fernandois.et al,J Endocrinol. ,Jan;232(1):97-105 EFFECT OF ADDING DRIED GINGER RHIZOMES TO DIETS ON SEMEN QUALITY AND FERTILITY RATE IN AGED LOCAL COCKS UNDER EGYPTIAN HOT Ezzat, W.et al,Egyptian Poultry Science Journal,Mar Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing HyunJun Park.et al,World J Mens Health,Oct 25 Citalopram Induces Reproductive Toxicity in Male Rats Ilgin S.et al,Birth Defects Res,Mar 31. Parabens Accelerate Ovarian Dysfunction in a 4-Vinylcyclohexene Diepoxide-Induced Ovarian Failure Model Jae-Hwan Lee .et al,Int. J. Environ. Res. Public Health,Feb 8 Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism Li J.et al,Stem Cell Res Ther.,Mar 9 Alteration in follistatin gene expression detected in prenatally androgenized rats Marziyeh Salehi Jahromi.et al,Gynecol Endocrinol,Feb 26 Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress Merve Baysa.et al,PLOS ONE,April 18 Investigating the prenatal exposure of hydro-alcoholic extract of ginger on the function of Pituitary-Gonad axis in male mature offspring rats Nasim Zamani .et al,BASIC AND LABORATORY RESEARCH,/ Therapeutic effect of curcumin against nicotine-induced reproductive dysfunction in male rats Neveen A. Salem.et al,JIPBS,/ How do vegetable oils (hazelnut and canola) affect the reproductive system in male rats? Bulent Kati.et al,Archivio Italiano di Urologia e Andrologia,Mar 31 Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats Ivana Jari.et al,Toxicology and Applied Pharmacology,Jan 15 Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats Tang YQ.et al,BMC Complement Altern Med,2 April Effects of chronic unpredictable mild stress on ovarian reserve in female rats: Feasibility analysis of a rat model of premature ovarian failure Xiao‑Yan Fu.et al,Molecular Medicine Reports,May 8 Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury Mohammadreza Gholami .et al,Journal of Complementary and Integrative Medicine,May 23 TOXIC EFFECTS OF TRAZADONE ON MALE REPRODUCTIVE SYSTEM VIA DISRUPTING HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS AND INDUCING TESTICULAR OXIDATIVE STRESS Sinem Ilgin.et al,/,/ Depletion of follicles accelerated by combined exposure to phthalates and 4-vinylcyclohexene diepoxide, leading to premature ovarian failure in rats Dinh NamTran.et al,Reproductive Toxicology,June 30 Comparative study of the effect of taurine, caffeine, and /or paracetamol on male fertility and teratogenicity in rats Asmaa M.Kandil.et al,Journal of Pharmacy and Pharmaceutics,43214 The Effect of Galega officinalis on Hormonal and Metabolic Profile in a Rat Model of Polycystic Ovary Syndrome Seyed Hosein Abtahi-Eivari.et al,International Journal of Women’s Health and Reproduction Sciences,July Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring Hassan I.H.El-Sayyad.et al,Reproductive Biology,July 10 Amelioration of diabetes‐induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment Tayebe Artimani.et al,ANDROLOGIA,July 18 Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats Zaid SSM, et al ,Journal of ovarian research,November 26 Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats Zaid SSM, et al ,Journal of ovarian research,November 26 Impact of Sleep Deprivation on the Hypothalamic-Pituitary-Gonadal Axis and Erectile Tissue Lee DS, et al,Journal of Sexual Medicine,January 7

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.