Rat Fibronectin,FN ELISA Kit

Rat Fibronectin,FN ELISA Kit

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Mfr.No.

CSB-E04553r

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibronectin 1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, OTTHUMP00000206762, OTTHUMP00000206767, cold-insoluble globulin, migration-stimulating fa

Recovery

The recovery of mouse MMP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-104
EDTA plasma (n=4)	91	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	87
	Range %	83-96
1:200	Average %	98
	Range %	92-105
1:400	Average %	102
	Range %	98-108
1:800	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.801	2.722	2.762	2.608
250	2.110	2.214	2.162	2.008
125	1.427	1.448	1.438	1.284
62.5	0.856	0.867	0.862	0.708
31.2	0.523	0.512	0.518	0.364
15.6	0.351	0.341	0.346	0.192
7.8	0.264	0.260	0.262	0.108
0	0.154	0.153	0.154	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04937

Applications	ELISA
Research Area	FN1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.