Rat FGF6(Fibroblast Growth Factor 6) ELISA Kit

Mfr.No.

ELK2488

Sensitivity

6.6 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

HBGF6; HST2; HST2 Oncogene; Heparin Secretory-Transforming Protein 2; Heparin-Binding Growth Factor 6

Recovery

Matrices listed below were spiked with certain level of recombinant FGF6 and the recovery rates were calculated by comparing the measured value to the expected amount of FGF6 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-101%	94%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	84-96%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	80-101%	87-96%	96-105%	95-103%
EDTA plasma(n=5)	85-105%	87-98%	91-102%	86-102%
Heparin plasma(n=5)	86-94%	93-102%	87-96%	92-105%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.058	1.975
500.00	1.587	1.504
250.00	1.216	1.133
125.00	0.933	0.850
62.50	0.518	0.435
31.25	0.395	0.312
15.63	0.156	0.073
0.00	0.083	0.000

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Cytokine;Tumor immunity;Infection immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205464	ELK2488	48T	$320		Add to Cart
BHE15205464	ELK2488	96T	$458		Add to Cart

Rat FGF6(Fibroblast Growth Factor 6) ELISA Kit

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